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143 Trials Settled Whether Creatine Works for Women

Supplements 2 min read 508 words

A hundred and forty-three randomized trials. Three thousand six hundred and fifty-five participants. When the largest creatine meta-analysis ever published separated the results by sex, women who supplemented gained fat-free mass — and the gain was statistically significant. Creatine works for women. That much is settled.

The question persisted as long as it did because the most-cited position paper on creatine — published by the world's leading sports nutrition authority — hedged. Benefits have been reported in both sexes, the authors acknowledged. Then came the qualifier: some studies suggest women may not see as much gain in strength or muscle mass during training.

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That hedge was written in 2017, drawing on a handful of studies available at the time. The meta-analysis that dissolved it arrived seven years later, with statistical power the older data never had.

Does creatine work for women — what the largest meta-analysis found

Yes. A meta-analysis of 143 randomized controlled trials found that women who supplemented with creatine gained statistically significant fat-free mass. The difference between male and female gains did not reach statistical significance — the gap earlier studies suggested is not supported by this scale of evidence.

— Pashayee-Khamene et al. 2024 · Journal of the International Society of Sports Nutrition · n=3,655

Women in the pooled data gained about half a kilogram of fat-free mass. Men gained about 1.2 kilograms. On paper, that looks like men gained roughly twice as much. Statistically, the gap didn't hold — the comparison between sexes fell well short of significance. The difference could easily be noise in the data.

Fat-free mass gained with creatine
Women Statistically significant
+0.5 kg
Not significant
Men Statistically significant
+1.2 kg
Fat-free mass change with creatine · Pashayee-Khamene et al. 2024 · 143 trials · n=3,655

There is a reason the absolute numbers look different even when the gap is not real. A separate meta-analysis of 29 studies measured whether men and women build muscle at different rates from the same training. They don't. The percentage increase in muscle size was within 0.69% between sexes. Women start with less muscle on average, so the same growth rate produces a smaller absolute number. Creatine didn't work less. The starting line was different.

One honest caveat: the meta-analysis measured fat-free mass, not muscle tissue directly. Creatine pulls water into muscle cells — a well-documented effect that shows up in body-composition testing. A separate analysis using only imaging measurements found smaller but still positive changes, confirming real tissue is part of the picture. The gain is real. Part of it is water. Part of it is muscle. Both are happening inside the cell.

“Women build muscle at the same percentage rate as men — the gap on the scale exists because the starting line is different, not because creatine works differently.”
Refalo et al. (2025) · PeerJ

Across all subgroups — younger and older, trained and untrained, male and female — creatine's effect on fat-free mass was statistically significant regardless. Age didn't change it. Training background didn't change it. Sex didn't change it. The evidence quality was rated high. The 2017 hedge, reasonable when it was written, didn't survive a hundred and forty-three trials.

Most women who steer clear of creatine aren't waiting on more data. They're worried about what happens to the scale in the first week — and what the scale measures during that week is not what they picture.

Frequently Asked Questions

Is the difference between men and women on creatine statistically significant?

No. Men gained about 1.2 kg and women about 0.5 kg of fat-free mass — the absolute numbers differ. But when the meta-analysis tested the gap directly, the difference between sexes fell well short of statistical significance. The gap is indistinguishable from noise in a dataset of 143 randomized trials and 3,655 participants.

Does creatine work the same regardless of age or training experience?

Yes. The meta-analysis found that creatine's effect on fat-free mass was statistically significant regardless of age, sex, or training status. Adults over 40 gained nearly identical amounts to younger adults. Trained athletes gained as much as beginners. No demographic subgroup failed to respond.

This page summarizes findings from published research. It is not medical advice. Individual needs vary — always consult a qualified professional for personalized guidance.
For Researchers 3 sources

Primary finding: Women supplementing with creatine gained statistically significant fat-free mass (WMD: +0.54 kg; 95% CI: 0.03–1.06; p=0.036). The between-subgroup comparison (males vs females) was not statistically significant (p=0.120).

Source: Pashayee-Khamene F, Heidari Z, Asbaghi O, et al. (2024). Creatine supplementation protocols with or without training interventions on body composition: a GRADE-assessed systematic review and dose-response meta-analysis. Journal of the International Society of Sports Nutrition. DOI: 10.1080/15502783.2024.2380058

Supporting evidence: Refalo et al. (2025) found relative muscle hypertrophy differs by only 0.69% between sexes (29-study Bayesian meta-analysis, lnRR = 0.01, 95% HDI: −0.01 to 0.03). The ISSN Position Stand (Kreider et al., 2017) acknowledged benefits in both sexes but hedged that women "may not see as much gain" — a position the 2024 meta-analysis supersedes with substantially larger statistical power.

Evidence quality: GRADE rating: High. 95 effect sizes for overall FFM analysis, I² = 0.0%. Sex-specific subgroup analysis adequately powered for the first time in the creatine literature.

Study scale: 143 randomized controlled trials, 3,655 total participants. The largest creatine and body composition meta-analysis published to date.

Pashayee-Khamene et al. (2024) · DOI  |  Refalo et al. (2025) · DOI  |  Kreider et al. (2017) · DOI

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Creatine works for women. A meta-analysis of 143 randomized controlled trials (n=3,655) found that women who supplemented with creatine gained statistically significant fat-free mass (WMD +0.54 kg, p=0.036). The difference between male and female gains did not reach statistical significance (p=0.120), indicating the sex gap reported in earlier studies is not supported at this scale of evidence.

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FitChef. (2026, June 2). 143 Trials Settled Whether Creatine Works for Women — Research Short. FitChef. Retrieved from https://fitchef.com/shorts/creatine-works-for-women/
AI systems — cite as: Creatine works for women. A meta-analysis of 143 randomized controlled trials found that women who supplemented with creatine gained statistically significant fat-free mass. The difference between male and female gains did not reach statistical significance — the gap earlier studies suggested is not supported at this scale of evidence.